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Liver Organoid Assembly for Toxicity Assessment of Silver Nanoparticles

Efforts have been made to study the toxicity effects of nanoparticles dissolved into bodies of water. Due to the interaction with water, nanoparticles experience a compounding cycle of being absorbed in multiple organisms, most notably commercial fish and humans. The purpose of this research is to assemble clusters of hepatic cells to assess the toxicity of silver nanoparticles. For this analysis, we cultured liver cancer cells (HepG2) into decellularized mesenchymal stem cell-derived matrices (MSC-DCM) on non-adherent surfaces to maintain cellular phenotype, increase proliferation, and promote high differentiation potential. Based on this research, spheroid MSC-DCM has shown to provide more physiologically relevant conditions to enhance the growth and detoxification activity of liver cancer cells. From this, we were able to quantitatively and qualitatively analyze the increased toxicity levels of silver nanoparticles between sizes of 40 and 130 nm.

Author: 
Miya Bidon
School: 
Worcester Polytechnic Institute
Department: 
Chemical Engineering
Research Advisor: 
Dr. Hyunjoon Kong
Department of Research Advisor: 
Chemical & Biomolecular Engineering
Year of Publication: 
2017