Evaluation of Intracameral Preservative-Free Lidocaine on Eyes of Dogs
This research focuses in the effects of intracameral injections of preservative-free lidocaine HCl on the eyes of dogs. Lidocaine is a local anesthetic frequently used in ophthalmic procedures in humans and animals. To conduct this research, sixteen dogs were randomly divided into two groups of eight dogs each. One eye of the dogs received a determined concentration of lidocaine and then the aqueous humor of each eye of the dogs was aspired and assayed with high-pressure liquid chromatography linked with mass spectrometry (HPLC-MS). In this particular research, the results obtained were used to develop a method to evaluate the concentration of lidocaine in the aqueous humor of dog's eyes. The results obtained were: a) the calibration curve for lidocaine in saline; b) the precision (0.7-6.01%) and accuracy (90-108%) ranges for lidocaine detection; and c) the peak areas for lidocaine in the aqueous humor falling into the 95% confidence interval of the saline samples. Chemical similarity between the saline and the aqueous humor reflected both matrices having the same mineral composition, and poor interference from chemicals such as proteins may explain the peak overlap seen in the results.
School:
Pontifical Catholic University of Puerto Rico at Ponce
Department:
Chemistry
Research Advisor:
Dr. Tomás Martín
Department of Research Advisor:
Veterinary Biosciences
Year of Publication:
2004