Differential Expression of Cyclins During Decidualization
Complex molecular bases of the interactions during the contact between the embryos' attachment to the uterus is an event regulated by the pathway of steroid hormones which stimulate the initiation, implantation, and establishes pregnancy. In receptive phase, the action of steroid hormones estrogen and progesterone are mediated by their nuclear receptor. The hormone bound receptors regulate the expression of specific gene networks in a variety of uterine cells during implantation. These genes in turn lead to differentiation of uterine cells into decidual cells, critical for implantation. To identify the genes regulated by the steroid hormone, we utilized a delayed implantation mouse model in which embryo attachment to the uterus is dependent on administration of estrogen to progesterone-primed pregnant uteri. In this research, the main motive is to analysis the expression of the cyclin D3, cyclin A, cyclin B and cyclin E during the phase of decidualization on the transformation of stromal cell to decidual cell. The cyclins are fundamental proteins in the regulation of the cell cycle and activate cyclin-dependent kinases (cdks). Using the artificial decidualization reaction, RNA isolation, reverse transcriptase- polymerase chain reaction (PCR), and immunocytochemistry we analyzed estrogen-regulated gene expression profiles in the delayed uteri. We observed that the lack of expression of cyclin B1 in PCR from unstimulated uterine tissue suggests the role for cyclin B1 in decidualization in compared with the B1 expression in stimulated uterine tissue. We can conclude that cyclin E2, cyclin D3, and cyclin A may be involved in the process of proliferation, helping in the transformation of stromal cells into decidual tissue. It has been proposed to quantify cyclins through Nothern Blot analysis in the future. An understanding of these molecules is critical for proving their involvement in implantation. Also, knowing which specific genes are expressed during the decidualization can contribute avoidance of early pregnancy loss and infertility.
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Year of Publication:
2004